Laboratory Furniture Fume Hood plan design and supply - Cartmay Industrial

(Seven) inoculation box 

Inoculation box is divided into two boxes inoculated solid and liquid inoculation. Inoculation is a solid box made ​​of wood and glass or plexiglass welded together by a closed casket. Divided into double and single operation box. Box vary, generally the box is about 143cm, width 86cm, total height 154cm, stand 76cm. The upper left and right sides of each box can be fitted with two leaves of glass sliding doors open and close, easy to strain out. The lower part of the window, respectively, with two round hole about 13cm in diameter, the center distance between the two holes of 52cm (shoulder width), the hole is equipped with elastic cuff, to prevent your hands when operating in the box, outside air into the tank the cause pollution. They sit opposite operation, his hands through the cuff extends into the box. Both sides of the box is also fitted with the best glass top boxes for wood or glass. Top box is equipped with ultraviolet germicidal lamps and fluorescent lighting each one. Cabinet install wood or glass can, but be careful sealed. 

Inoculated liquid tank is designed for transposing liquid spawn and design. Bacteria than solid elongated box, unilateral two operations. Features tracks and ultraviolet light, both ends of the box to open a high 25cm, width 10cm rectangular outlet, convenient bacteria and out the hole with a small door. Import and export Xiachu set a vapor source, import and export of seal inoculation with steam to prevent the bacteria entering the box. The back of the box has moved to take over the liquid bacteria can enter the hole. 

Inoculation box sterilized with ultraviolet irradiation 30min. If there is no ultraviolet light, can be used formaldehyde and potassium permanganate (formaldehyde 10 ~ 14mL / m3 + potassium permanganate 5 ~ 7g / m3 space) fumigation than 30min. When used, the first vaccination required items and tools into the box, and then fumigated and UV sterilization agents, and then aseptically inoculated. 

Simple inoculation box structure, low cost, easy sterilization, easy to operate, and people operating outside the box, can work at higher temperatures. The disadvantage is that the medium access time and labor, are required prior to each vaccination sterilization. 

(Eight) refrigerator 

Microbiological laboratory refrigerators are mainly two types: ordinary refrigerators and cryogenic refrigerator. Ordinary refrigerator generally have two cabinets, namely fresh Tibetan cabinets and freezers, temperatures were 4 ℃ and -20 ℃; cryogenic refrigerator temperature control at -40 ~ -80 ℃. They can be used for the Deposit of Microorganisms. Fresh Tibetan cabinet 

 

Commonly used to save slant bacteria, preserved time in three months. More than 3 months, the slope will become dry, and therefore needs to transfer species. If you want to save the species for a long time, you need to go through post-processing, storage in an ordinary refrigerator freezer or cryogenic refrigerator, they preserved a long time, generally more than a year. 

 

 

Microbiology Laboratory Management 

 

A laboratory management system 

1. Laboratory instruments should be developed with the management, use the system, drug management, use the system, glassware management, the use of the system, and in accordance with requirements of safety systems and environmental conditions, this room staff should be strictly controlled, carefully executed. 

2. Into the lab must wear overalls, non-laboratory personnel shall not enter the laboratory, strict implementation of safety procedures. 

3. Laboratory items neatly, agents regularly checked and have clear labeling, instrumentation regular inspection, maintenance, overhaul, is strictly prohibited in the refrigerator for storing and processing of private food. 

4. Various equipment should be established for claiming consumption records, precious instrument has to use records, broken missing report should be completed; medicines, equipment, bacteria are not allowed to borrow without the approval and transfer, and more shall come privately. 5. Smoking is prohibited in the laboratory, dining parlor, noise, may not bring personal items in the laboratory, water and electricity before leaving the laboratory carefully check for toxic, hazardous, flammable, pollution, corrosive substances and waste materials should be relevant Requests the Executive. 

6. Strict implementation of the person in charge of the system, report the problem immediately. 

Second, the instrument is equipped, management and use of the system 

1. Food microbiology laboratories should have the following equipment: incubator, pressure cooker, ordinary refrigerator, cold refrigerator, anaerobic training equipment, microscopes, centrifuges, clean units, oscillators, general balance, one-thousandth of balance, oven, freeze-dried equipment, homogenizer, thermostatic water bath, colony counter, incubator, potential pH meter, high-speed centrifuges. 

2 laboratory instrument used, the container shall comply with the standard requirements to ensure accurate and reliable, all measuring instruments shall be certified by the measurement department before using. 

3. Laboratory instruments placed reasonable, there is someone keeping valuable instrument to establish the instrument archives, and with methods of operation, maintenance, repair, and use of manual registration of this, do regular maintenance, maintenance and inspection, precision instruments are not free to move, if damaged when in need of repair, not secretly dismantled, should write the report, notify managers, by the manager agreed to fill repair application, send equipment maintenance department. 

4. Various instruments (refrigerator, temperature except me), immediately after use to cut off the power knob recovery homing, to be carefully examined before leaving. 

5. All instruments and equipment without equipment managers agree not borrow, after the registration of the use by content were registered. 

6. Equipment should be kept clean, it should normally instrument shroud. 

7. When using the instrument should be carried out strictly in accordance with the rules, in violation of the rules of the instrument due to mismanagement resulting in equipment damage, the parties concerned should be held accountable. 

Third, drug management, use system 

1. The room inspection tasks in accordance with the development of various pharmaceutical agents procurement plan, include full name, unit, quantity, purity, packaging specifications, date of manufacture, and establishing accounts, after someone managed to reclaim every six months to make the consumption table, and the remaining inventory drugs. 

2. Drug agents display tidy, orderly place, away from light, moisture, air dry, bottle label intact, locked storage of highly toxic drugs, flammable, volatile, corrosive species separate storage. 

3. Consuming drugs reagents, please fill out the required lead single, and room by using the person in charge to sign any person not entitled to privately 

Lend or feed drug agents, the unit Branch, Room occasionally signed by the department head of interlibrary loan from other units when needed. 4. Weigh Drug agents should be operating specifications, after using cover, if necessary, seal or black paper parcel, do not use expired or deteriorated drugs. 

Fourth, glassware management, use the system 

1. According to the requirements of test items, glassware declaration procurement plan, detailing the specifications, origin, quantity, requirements, hard neutral glass instrument should be verified by qualified metering. 

2. Large containers establish accounts, inventory once a year, usually low-value consumable inventory at any time after completing the registration utensils damage loss. 

3. After glassware dirt should be removed before use, and use cleaning fluid or 2% diluted hydrochloric acid solution soak for 24 h, rinse with water reserve. 

4. After cleaning utensils to use at any time after the contamination should be strictly autoclaving, not indiscriminate disposal of litter. 

Fifth, security system 

1. Into the laboratory work clothes, hats, shoes must be worn neatly. 

2. During high pressure, drying, disinfection and other work, the staff are not allowed from the scene, carefully observe the temperature, time, distilling volatile, flammable liquid when you are not allowed to directly heat the water bath should be set, such as produced during the test in the cabinet should avoid poison gas operations. 

3. When the draw is strictly prohibited drugs and bacteria directly with the mouth, according to the aseptic operation, such as bacteria occur, the pathogen spill containers, shall immediately be thoroughly disinfected with an effective disinfectant, before leaving the scene safe handling. 4. After work, wash hands with soap and water, if necessary, can Bromogeramine, peracetic acid foam hand, then rinse with water, clothes should be cleaned regularly to keep clean and, if necessary, autoclaved. 

5. Experiment is completed, the immediate cleaning up the site and experimental equipment for contamination infected items, disinfection and sterilization process. 6. Daily work, especially around holidays, carefully check the water, electricity and equipment being used, shut the doors and windows before leaving. 

Six, environmental conditions 

1. The laboratory should always be kept clean, cleaning should be carried out every day to work on finishing, desk cabinets and other surfaces should be 

Wipe with a disinfectant every day, to keep clean, to prevent contamination. 2. The laboratory should orderly, shall not be stored outside the laboratory and personal belongings, equipment and other laboratory supplies to put reasonable, and have a fixed position. 3. Keep in laboratory health not throw confetti and other debris, test the waste to be used in a fixed box down the barrel, and timely manner. 

4. The laboratory should have excellent light conditions and lighting. 

5. Laboratory work surface should be level and no leakage, walls and floors should be smooth and easy to clean. 6. Laboratory layout to be reasonable, generally between laboratories should prepare and sterile room, sterile room should have good ventilation conditions, such as installation of air conditioning equipment and filtration equipment, sterile indoor air testing should be basically sterile. 7. Do not use the laboratories for conference rooms and other recreational activities and learning spaces.